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bio rad miniopticon real time pcr system  (Bio-Rad)


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    Bio-Rad bio rad miniopticon real time pcr system
    Bio Rad Miniopticon Real Time Pcr System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 9490 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+rad+miniopticon+real+time+pcr+system/SsoFast+Eva+Green+Supermix/bio_rxiv__64898__2026__02__21__707236-94-14-14
    Average 99 stars, based on 9490 article reviews
    bio rad miniopticon real time pcr system - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: The transcription factor masculinizer in sexual differentiation and achieved full functional sex reversal in prawn
    Article Snippet: .. The qPCR reactions were performed on the Bio-Rad MiniOpticon Real-Time PCR System using SYBR Premix Ex Taq (TaKaRa Bio Inc., Japan) and specific primers, Mr-Masc-qF and Mr-Masc-qR ( ). ..

    Article Title: Hypoxia-induced downregulation of microRNA-186-5p in endothelial cells promotes non-small cell lung cancer angiogenesis by upregulating protein kinase C alpha
    Article Snippet: The further qRT-PCR assay was performed with 100 ng of cDNA using the miScript and QuantiTect SYBR Green PCR Kit (Qiagen) for the detection of miRNA and mRNA, respectively. .. A total of 40 cycles were performed in the Bio-Rad MiniOpticon Real-Time PCR System (Bio-Rad Laboratories), and the cycling parameters were as follows: initial activation at 95°C for 15 min, denaturation at 94°C for 15 s, annealing at 55°C for 30 s, and extension at 70°C for 34 s (miScript SYBR Green PCR Kit) or extension at 72°C for 30 s (QuantiTect SYBR Green PCR Kit). ..

    Article Title: Epicatechin and β-Glucan from Whole Highland Barley Grain Synergistic Benefit on Attenuating Hyperglycemia via Improving Hepatic Glucose Metabolism in Diabetic C57BL/6J Mice.
    Article Snippet: Our previous study proved that epicatechin (EC) and β-glucan (BG) from whole-grain highland barley synergistically modulate glucose metabolism in insulin-resistant HepG2 cells.. However, the main target and the mechanism underlying the modulation of glucose metabolism in vivo remain largely unknown.. In this study, cell transfection assay and microscale thermophoresis analysis revealed that EC and BG could directly bind to the insulin receptor (IR) and mammalian receptor for rapamycin (mTOR), respectively.

    Article Title: Epicatechin and β-glucan from whole highland barley grain ameliorates hyperlipidemia associated with attenuating intestinal barrier dysfunction and modulating gut microbiota in high-fat-diet-fed mice.
    Article Snippet: Hyperlipidemia is associated with intestinal barrier dysfunction and gut microbiota dysbiosis.. Here, we aimed at investigating whether epicatechin (EC) and β-glucan (BG) from whole highland barley grain alleviated hyperlipidemia associated with ameliorating intestinal barrier dysfunction and modulating gut microbiota dysbiosis in high-fat-diet-induced mice.. It was observed that EC and BG significantly improved serum lipid disorders and upregulated expression of PPARα protein and genes.


    Article Title: Mitochondrial structural and functional defects in the Drosophila melanogaster model of PLA2G6 Associated Neurodegeneration (PLAN)
    Article Snippet: .. Quantitative PCR (10 μL) was performed using SsoFast EvaGreen Supermix (Bio-Rad, 172-5201) on a Bio-Rad MiniOpticon Real-Time PCR System. ..

    Article Title: Transcriptional Regulatory Network of the Embryonic Diapause Termination Process in Artemia
    Article Snippet: RNA was extracted with TRIzol reagent (Invitrogen, San Diego, CA, USA) according to the manufacturer’s instructions; the total RNA (1 μg) was reverse transcribed in a 10 μL reaction containing oligo (dT) primers and M-MLV Reverse Transcriptase (TaKaRa, Shiga, Japan). .. PCRs were performed using SYBR Green (TOYOBO, Osaka, Japan) on the Bio-Rad MiniOpticon real-time PCR system in triplicate. ..

    Activation Assay:

    Article Title: Hypoxia-induced downregulation of microRNA-186-5p in endothelial cells promotes non-small cell lung cancer angiogenesis by upregulating protein kinase C alpha
    Article Snippet: The further qRT-PCR assay was performed with 100 ng of cDNA using the miScript and QuantiTect SYBR Green PCR Kit (Qiagen) for the detection of miRNA and mRNA, respectively. .. A total of 40 cycles were performed in the Bio-Rad MiniOpticon Real-Time PCR System (Bio-Rad Laboratories), and the cycling parameters were as follows: initial activation at 95°C for 15 min, denaturation at 94°C for 15 s, annealing at 55°C for 30 s, and extension at 70°C for 34 s (miScript SYBR Green PCR Kit) or extension at 72°C for 30 s (QuantiTect SYBR Green PCR Kit). ..

    SYBR Green Assay:

    Article Title: Hypoxia-induced downregulation of microRNA-186-5p in endothelial cells promotes non-small cell lung cancer angiogenesis by upregulating protein kinase C alpha
    Article Snippet: The further qRT-PCR assay was performed with 100 ng of cDNA using the miScript and QuantiTect SYBR Green PCR Kit (Qiagen) for the detection of miRNA and mRNA, respectively. .. A total of 40 cycles were performed in the Bio-Rad MiniOpticon Real-Time PCR System (Bio-Rad Laboratories), and the cycling parameters were as follows: initial activation at 95°C for 15 min, denaturation at 94°C for 15 s, annealing at 55°C for 30 s, and extension at 70°C for 34 s (miScript SYBR Green PCR Kit) or extension at 72°C for 30 s (QuantiTect SYBR Green PCR Kit). ..

    Article Title: Epicatechin and β-Glucan from Whole Highland Barley Grain Synergistic Benefit on Attenuating Hyperglycemia via Improving Hepatic Glucose Metabolism in Diabetic C57BL/6J Mice.
    Article Snippet: Our previous study proved that epicatechin (EC) and β-glucan (BG) from whole-grain highland barley synergistically modulate glucose metabolism in insulin-resistant HepG2 cells.. However, the main target and the mechanism underlying the modulation of glucose metabolism in vivo remain largely unknown.. In this study, cell transfection assay and microscale thermophoresis analysis revealed that EC and BG could directly bind to the insulin receptor (IR) and mammalian receptor for rapamycin (mTOR), respectively.

    Article Title: Epicatechin and β-glucan from whole highland barley grain ameliorates hyperlipidemia associated with attenuating intestinal barrier dysfunction and modulating gut microbiota in high-fat-diet-fed mice.
    Article Snippet: Hyperlipidemia is associated with intestinal barrier dysfunction and gut microbiota dysbiosis.. Here, we aimed at investigating whether epicatechin (EC) and β-glucan (BG) from whole highland barley grain alleviated hyperlipidemia associated with ameliorating intestinal barrier dysfunction and modulating gut microbiota dysbiosis in high-fat-diet-induced mice.. It was observed that EC and BG significantly improved serum lipid disorders and upregulated expression of PPARα protein and genes.

    Article Title: Transcriptional Regulatory Network of the Embryonic Diapause Termination Process in Artemia
    Article Snippet: RNA was extracted with TRIzol reagent (Invitrogen, San Diego, CA, USA) according to the manufacturer’s instructions; the total RNA (1 μg) was reverse transcribed in a 10 μL reaction containing oligo (dT) primers and M-MLV Reverse Transcriptase (TaKaRa, Shiga, Japan). .. PCRs were performed using SYBR Green (TOYOBO, Osaka, Japan) on the Bio-Rad MiniOpticon real-time PCR system in triplicate. ..

    Polymerase Chain Reaction:

    Article Title: Hypoxia-induced downregulation of microRNA-186-5p in endothelial cells promotes non-small cell lung cancer angiogenesis by upregulating protein kinase C alpha
    Article Snippet: The further qRT-PCR assay was performed with 100 ng of cDNA using the miScript and QuantiTect SYBR Green PCR Kit (Qiagen) for the detection of miRNA and mRNA, respectively. .. A total of 40 cycles were performed in the Bio-Rad MiniOpticon Real-Time PCR System (Bio-Rad Laboratories), and the cycling parameters were as follows: initial activation at 95°C for 15 min, denaturation at 94°C for 15 s, annealing at 55°C for 30 s, and extension at 70°C for 34 s (miScript SYBR Green PCR Kit) or extension at 72°C for 30 s (QuantiTect SYBR Green PCR Kit). ..

    Synthesized:

    Article Title: Epicatechin and β-Glucan from Whole Highland Barley Grain Synergistic Benefit on Attenuating Hyperglycemia via Improving Hepatic Glucose Metabolism in Diabetic C57BL/6J Mice.
    Article Snippet: Our previous study proved that epicatechin (EC) and β-glucan (BG) from whole-grain highland barley synergistically modulate glucose metabolism in insulin-resistant HepG2 cells.. However, the main target and the mechanism underlying the modulation of glucose metabolism in vivo remain largely unknown.. In this study, cell transfection assay and microscale thermophoresis analysis revealed that EC and BG could directly bind to the insulin receptor (IR) and mammalian receptor for rapamycin (mTOR), respectively.

    Article Title: Epicatechin and β-glucan from whole highland barley grain ameliorates hyperlipidemia associated with attenuating intestinal barrier dysfunction and modulating gut microbiota in high-fat-diet-fed mice.
    Article Snippet: Hyperlipidemia is associated with intestinal barrier dysfunction and gut microbiota dysbiosis.. Here, we aimed at investigating whether epicatechin (EC) and β-glucan (BG) from whole highland barley grain alleviated hyperlipidemia associated with ameliorating intestinal barrier dysfunction and modulating gut microbiota dysbiosis in high-fat-diet-induced mice.. It was observed that EC and BG significantly improved serum lipid disorders and upregulated expression of PPARα protein and genes.

    Quantitative RT-PCR:

    Article Title: Epicatechin and β-Glucan from Whole Highland Barley Grain Synergistic Benefit on Attenuating Hyperglycemia via Improving Hepatic Glucose Metabolism in Diabetic C57BL/6J Mice.
    Article Snippet: Our previous study proved that epicatechin (EC) and β-glucan (BG) from whole-grain highland barley synergistically modulate glucose metabolism in insulin-resistant HepG2 cells.. However, the main target and the mechanism underlying the modulation of glucose metabolism in vivo remain largely unknown.. In this study, cell transfection assay and microscale thermophoresis analysis revealed that EC and BG could directly bind to the insulin receptor (IR) and mammalian receptor for rapamycin (mTOR), respectively.

    Article Title: Epicatechin and β-glucan from whole highland barley grain ameliorates hyperlipidemia associated with attenuating intestinal barrier dysfunction and modulating gut microbiota in high-fat-diet-fed mice.
    Article Snippet: Hyperlipidemia is associated with intestinal barrier dysfunction and gut microbiota dysbiosis.. Here, we aimed at investigating whether epicatechin (EC) and β-glucan (BG) from whole highland barley grain alleviated hyperlipidemia associated with ameliorating intestinal barrier dysfunction and modulating gut microbiota dysbiosis in high-fat-diet-induced mice.. It was observed that EC and BG significantly improved serum lipid disorders and upregulated expression of PPARα protein and genes.

    Isolation:

    Article Title: Epicatechin and β-glucan from whole highland barley grain ameliorates hyperlipidemia associated with attenuating intestinal barrier dysfunction and modulating gut microbiota in high-fat-diet-fed mice.
    Article Snippet: Hyperlipidemia is associated with intestinal barrier dysfunction and gut microbiota dysbiosis.. Here, we aimed at investigating whether epicatechin (EC) and β-glucan (BG) from whole highland barley grain alleviated hyperlipidemia associated with ameliorating intestinal barrier dysfunction and modulating gut microbiota dysbiosis in high-fat-diet-induced mice.. It was observed that EC and BG significantly improved serum lipid disorders and upregulated expression of PPARα protein and genes.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Procyanidin B1 and Coumaric Acid from Highland Barley Alleviated High-Fat-Diet-Induced Hyperlipidemia by Regulating PPARα-Mediated Hepatic Lipid Metabolism and Gut Microbiota in Diabetic C57BL/6J Mice
    Article Snippet: .. RT-PCR was conducted on a Bio-Rad MiniOpticon real-time PCR system (Bio-Rad, CA, USA) by using cDNA, forward and reverse primers, and SYBR Green Supermix (TIANGEN Biotech, Beijing, China). ..



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